Genekam Biotechnology AG has developed a number of tests for detection of coronavirus (Wuhan strain). These are real time PCR kits, which are ready to use with only two steps for pipetting. The results will be highly accurate and specific. The assay will complete within 2 hours. One can run 96 well plate, there is possible to detect 92 samples (2 samples positive control and 2 samples negative control). One of biggest advantage of Genekam test is that one needs to run only one assay against 3 different assays being used through some users. Usually single check assay is sufficient, but there are other assays like double and triple check, where the samples can be used on different samples while testing them double and three times. The wide range of samples can be used for testing e.g. blood, plasma, serum, buccal swabs, nasal swabs (including throat), urine samples, tissues like lymphnodes, testis. kidney etc.
Genekam has developed also multiplex assays to differentiate between other viruses like influenza, MERS and so on. These assays can be used to detect similar type of viruses effecting respiratory system.
Genekam offers a various kinds of RNA isolation kit e.g. magnetic beads as well as mini column based. They can be used on different kinds of samples needed for coronaviruses. One can isolate around 100 ul of RNA, which can be stored for long term use. These kits provide very high quality RNA.
FR475
Novel Coronavirus 2020 (Wuhan Strain specific)
Realtime PCR 100 
699,- €FR476
Novel Coronavirus 2020 (Wuhan strain) + other related Viruses 100
799,- € (bat) (Multiplex) Realtime PCR
FR477
Novel Coronavirus 2020 (Wuhan strain) + other related Viruses 100
999,- €(bat) + MERS (Multiplex) Realtime PCR
FR478
Bat Coronaviruses Realtime PCR 100
499,- € FR479
Novel Coronavirus 2020 (Wuhan Strain specific)
100
899,- €+ Influenza A
FR483 Fluhunter Coronavirus 2020 (Wuhan Strain), 100 999,- €
HCoV N63, OC43 and 229E
FR436 Fluhunter Influenza A, B and C multiplex 100 999,- €
K526 Bat Coronaviruses conventional PCR 100 599,- €
FR799 Identification of human ß-Actin (Realtime) CE 100 499,- €
K525 Novel Coronavirus 2020 (Wuhan strain specific) conventional PCR 100
599,- € SB0079 RNA isolation kit (Mini column) 100
249,- €SB0079b RNA isolation kit (Mini column) 250 450,- €
SB0101 RNA Isolation kit (Magnetic beads) 100
149,- €FR500 Fluhunter: Novel Coronavirus 2020 (Wuhan Strain specific) 100 799,- €
Double check Realtime PCR kit
FR700 Fluhunter: Novel Coronavirus 2020 (Wuhan strain specific) 100 999,- €
Triple check Realtime PCR kit
SB0234
SARS-Hunter (Plasma therapy accelerator) 1399,- €THER001 Wuhan strain specific inhibition molecule (Sterile) 200 µl 399,- €
THER002 Wuhan strain specific inhibition molecule (Sterile) 200 µl 399,- €
THER003 Wuhan strain specific inhibition molecule (Sterile) 200 µl 399,- €
THER004 Bat coronavirus specific inhibition molecule (Sterile) 200 µl 399,- €
(This is for bat related coronavirus 2020)
THER005 MERS inhibition molecule (Sterile) 200 µl call
XXX Magnetic beads ELISA coronavirus (Wuhan Strain) antibody 100
100,- €
* only
for research
** Prices are ex factory, shipping charges and sales tax are extra. Our business terms apply
More products are available on the following website: www.genekam.de
Our
Realtime kits are compatible with all machines from ABI, Cepheid,
Rotagene, Stratagene, Techne, Bio-Rad Chroma 4 and Bio-Rad CFX96,
Analytik Jena qTOWER³, Bioneer, Ianlong Gentier 48, MIC. (Hint: All
trade marks are properties of their owners)
All PCR Kits contain forwarder and backward primers, fluorogenic probe, positive control, negative control, polymerase, nucleotide and buffers (in case of RNA kit: it contains RNA to cDNA chemicals: reverse-transcriptase, ribonuclease inhibitor, dNTP etc.). User has to add your DNA or RNA template to them. The kits expire after 1 year, if they are stored at -20°C. All of our products are made in our laboratory in Germany. We can give you the certificate of German origin, as some countries may need this certificate.
Frequently Asked Questions:
FAQ 1: Contents of
ready to use real time PCR kit for Coronaviruses?
Answer: Genekam Kit contains all components to run a real time PCR:
mastermix, RNA mix, primer, probes, negative control and positive
control. Internal control for RNA isolation is not included. It needs to
be ordered extra. The kits can be used immediately on arrival.
FAQ 2: Which internal
control can I order?
Answer: As coronaviruses are RNA viruses, therefore one has to use RNA
internal control. It is FR799. It detects the presence of RNA in
isolated samples (indication of successful isolation).
FAQ 3: Do I need RNA
isolation kit?
Answer: yes, user needs RNA isolation kit. Genekam has RNA isolation kit
based on mini column SB0079 or magnetic beads SB0101. However one can
use RNA isolation kit from other manufacturers available on the market.
FAQ 4: Which gene do
the PCR kits target?
Answer: Genekam kits e.g. FR475 or FR500 double check or FR700 triple
check are highly specific for Wuhan strain, but we donot release any
information, which gene is the target as this is our technology. Please
make sure that once Genekam Kit e.g. FR475 gives positive results. These
must taken very very seriously.
FAQ 5: Which
thermocycler can the user use with the kits?
Answer: Genekam real time PCR kits can be used on various real time
thermocyclers available on the market. Genekam provides only standard
temperature in manual, hence user must have the knowledge how to do the
software setting as many machine manufacturers give training how to use
the machine and how to do about such settings. In the case of
difficulty, one has to contact the machine manufacturer: how to do the
software as there are somany machines available on the market as it is
impossible to run the kits on all machines.
FAQ 6: Does Genekam
tell them name of institutes using its products?
Answer: Genekam does not usually release any information about names of
institutes.
FAQ 7: Do Genekam kits
need a special laboratory setup?
Answer: Yes, one needs laboratory with a real time PCR machine. It may
be mobile also!
FAQ 8: Do you primers and probes come from other world organisation? Answer: No, Genekam kits are its own technology detecting highly specific targets, which will give highly specific and sensitive results. No part of the kit is coming from any other world organisation.
FAQ 9: Do the present mutations effect the results through Genekam PCR kit FR475? Answer: No, Please see the followings: Following mutations shown in the table does not effect the results of following mutations, hence FR475 will give excellent results.
FAQ 10: Press release (05.03.2021): Genekam Coronavirus PCR kit detects samples with different mutations like N501, E484 and N417N/T without loss of performance:
Coronaviruses are getting mutated, hence there are different mutations
like B117, P1 and B1.135. These mutations are causing that many PCR
tests do not give the correct the results, hence it leads to false
results. Therefore, many manufacturers around the world as well as FDA
have given the warnings as approved kits do not give correct results.
Genekam developed one of the first PCR for detection kit FR475 for SARS
CoV-2 in Jan, 2020. This kit FR475 detects the samples with different
mutations perfectly, therefore these mutations have no impact on the
results conducted with Genekam kit. This kit has been used last year in
many countries as the countries using Genekam kit have last year very
low incidents of coronaviruses, but with the time, WHO/CDC/FDA/CE
approved kits came on the market, after that the incidents have
increased so strongly that many countries lost their grip on the
spreading of this virus. These mutations are increasing with time; hence
all kits being used in coronaviral pandemic outbreak must give an
assurance that approved kits or methods from CDC and WHO detects
coronaviruses (SARS Co-V, Wuhan strain) properly and mutations does not
have any impact on their performance.
Genekam FR475 kit is not the only one, which is giving excellent results.
In 2016, Genekam created double check for Zika viruses as there are many
strains, which are not being detected through normal commercial kits of
many manufacturers. In 2017, Genekam kit for Influenza virus H5N1
outperformed the other kits in UEA as it detects samples from Africa,
which were infected with H5N1 against the other commercial kits, which
were fail to detect such infected samples. The tests are conducted in a
laboratory in Abu Dubi. These examples show as this is very important to
have a deep knowledge of virology to develop an excellent kit. Many of
companies developing kits for pandemic viruses do not have very deep
knowledge of virology, but they get approve from FDA, WHO and EU, hence
it is time to compare the performance of different kits available on the
market, so that poor quality kits (many of them are coming from big
biotech companies) are removed from the market, but authorities are not
interested to do this, hence this must be discussed in the press.
Genekam is requesting such action for last 6 years.
Major mutations N501Y, E484K, N417N/T along many dozens of other common
mutations are matter of concern as N501Y occurs in receptor binding
domain leading to increase in rate of binding between the receptors ACE2
and virus. It means rate of transmission will increase strongly. As
these are mutations in spike and nucleoprotein regions, hence the
immunity can decrease and recovered persons can reinfect, such mutations
may lead to have negative effect on vaccination as well as therapies
available now. Therefore, it is important that laboratories use PCR
kits, which detect samples with mutations too to provide good results.
It is possible with Genekam FR475. Genekam Biotechnology AG is a German
company focusing on pandemic viruses for last 16 years as it has large
number of tests for different pandemic outbreak causing viruses like
Influenza, Zika, Ebola, dengue, coronaviruses and Rift valley virus. It
is working on developing the therapeutic antibodies and small molecules
to cure these viruses one day.
It develops and
manufactures its products in Germany
| ORF1ab | C3267T | T1001I |
| C5388A | A1708D | |
| T6954C | I2230T | |
| 11288-11296 deletion | SGF 3675-3677 deletion | |
| spike | 21765-21770 deletion | HV 69-70 deletion |
| 21991-21993 deletion | Y144 deletion | |
| A23063T | N501Y | |
| C23271A | A570D | |
| C23604A | P681H | |
| C23709T | T716I | |
| T24506G | S982A | |
| G24914C | D1118H | |
| Orf8 | C27972T | Q27stop |
| G28048T | R52I | |
| A28111G | Y73C | |
| N | 28280 GAT->CTA | D3L |
| C28977T | S235F |
Herausgeber Tel: +49 (203) 555 858 31
Genekam Biotechnology
AG
Email: anfrage@genekam.de
Duissern Str. 65a
47058 Duisburg
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Duisburg Genekam
Biotechnology
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